HALO™ UHPLC Columns

- 2.7 µm semi-porous particles manufactured with Fused-Core™ technology
- The same high efficiency as with sub 2 µm particles but with considerable lower backpressure
- Extremely fast separations – even with a traditional HPLC-system
- Very robust columns
- Can be used at extreme pressures
- 2 µm frits in the column can be used because of tight particle size distribution
- Developed by Dr. Jack Kirkland, one of the pioneers in chromatography
Biotech AB is responsible for the sales of the HALO™-columns in the Nordic market (Sweden, Norway, Denmark, Finland)

The HALO 5 µm particles are exciting and novel because they can be packed into columns that will change the way you will think about HPLC by providing the following benefits:
- The highest plate counts versus any other 5-micron column you can buy (> 150,000 plates per meter)
- Equivalent or higher number of plates as most 3-micron columns with one-half the pressure
- The ruggedness of 5-micron columns (stable packed beds, resistance to pluggage) with the performance of 3-micron columns
- Improved productivity resulting from having the same thin porous layer as 2.7-micron HALO columns allowing high efficiencies at faster flow rates
- Low back-pressure well suited to legacy HPLC instruments
- Scalable from UHPLC separations developed on 2.7-micron HALO columns
- Available in lengths from 20-250mm and ID 2.1, 3.0 and 4.6mm
We offer 5 different phases in the 5 micron particle dimension These are:
- C18
- PFP
- Phenyl-Hexyl
- C8
- ES-CN
- Cephalosporins on Penta-HILIC and HILIC
- Fibrates on C18 ACN
- Fluoroquinolones on Phenyl-Hexyl
- Penicillins on HALO ES-CN
- Phenyl Ureas on ES-CN
- Statins on HALO C8 MeOH
- Steroids on PFP C18 MeOH 047-STR
- Xanthines on RPA ACN 048-XA
- Phenyl ureas on HALO-5 C18 vs 3 um C18
- Phenyl Ureas on ES-CN
- Penicillins on HALO ES-CN
- Halo 5 vs 5u totally porous-NSAIDS
- HALO 2.7 u C18 vs. HALO 5 u C18
- Fluoroquinolones on Phenyl-Hexyl
- Fibrates on C18 ACN
- Cephalosporins on Penta-HILIC and HILIC
- Apotransferrin Tryptic Digest on HALO Peptide ES-C18
- 105-PR IgG2-B Antibody separation on HALO Protein C4
- 101-B Nucleotides on HALO Penta-HILIC
- Xanthines on RPA ACN 048-XA
- Steroids on PFP C18 MeOH 047-STR
- HALO Application notes
- Anesthetics on HALO 2 Penta HILIC
- Benzodiazepines on HALO 2 Phenyl Hexyl
- Steroids on HALO 2 PFP